In vitro and in vivo study approaches

Discover what we do in the laboratory

Molecular Biology Techniques

  • DNA recombination assays, cloning and in situ mutagenesis.
  • DNA Sequencing: Whole-Exome and Nanopore Sequencing
  • RNA Biology: semiquantitative and quantitative PCR; genome-wide analysis of RNA (RNAseq), UV- Cross-linking and Immunoprecipitation (CLIPseq) and Chromatin Immunoprecipitation (ChIPseq)-sequencing datasets.
  • In silico analysis of public datasets, in vivo splicing and 3’RACE assays.
  • Analyses of nascent RNA transcripts and RNA polymerase II processivity.
  • Protein Biology: western blot, transient and stable depletion/overexpression of selected genes, luciferase assay, Protein-DNA (ChIP), Protein-RNA (RNA-pulldown, CLIP, RNA immunoprecipitation), and Protein-Protein (Protein gradient fractionation, yeast and mammal two-hybrid, GST-protein pulldown, Co-immunoprecipitation) interaction. Luciferase assay.

Cell Biology and Histology Techniques

  • Cell culture,
  • proliferation, clonogenic assays,
  • Invasion and chemotherapy response assays,
  • Flow cytometry,
  • Immunofluorescence,
  • Fluorescence In Situ Hybridization (FISH)
  • Organ inclusion and H&E staining,
  • Immunohistochemistry
  • Meiotic chromosome spreads
  • Fluorescence microscopy and deconvolution
  • Bright field microscopy

In vivo Studies on Animal Models

  • Animal manipulation from embryonal to adult stage.
  • Generation of Knock-in, knock-out mouse models and their phenotypic characterization
  • Generation of xenograft models for the in vivo study of cancers progression and response to chemotherapy treatments.